Journal: Translational neuroscience

Article Title: Decreased MiR-30a promotes TGF-β1-mediated arachnoid fibrosis in post-hemorrhagic hydrocephalus.

doi: 10.1515/tnsci-2020-0010

Figure Lengend Snippet: Figure 6: In the in vitro model of arachnoid cell fibrosis, the expression of miR-30a decreased, and the expression of TRAF3IP2 was upregulated. (a) Differential mRNA expression of miR-30a, TRAF3IP2, and TGF-β1 in arachnoid cells between the fibrotic group and control group (**P < 0.01 compared with the control group). (b) Differential protein expression of TRAF3IP2, TGF-β1, and α-SMA in arachnoid cells between the fibrotic and control groups (**P < 0.01 compared with the control group; *P < 0.05 compared with the control group). (c) Differential content of collagen I and collagen III in the model of arachnoid cell fibrosis (**P < 0.01 compared with the control group).

Article Snippet: ELISA kit was used to detect the TGF-β1 level (EK0514, Wuhan Boster Biological Technology, Ltd, China), collagen type I level (CSB-E08084r, CUSABIO, China), and collagen type III level (CSB-E07924r, CUSABIO, China) following the protocols constructions.

Techniques: In Vitro, Expressing, Control

Journal: Translational neuroscience

Article Title: Decreased MiR-30a promotes TGF-β1-mediated arachnoid fibrosis in post-hemorrhagic hydrocephalus.

doi: 10.1515/tnsci-2020-0010

Figure Lengend Snippet: Figure 7: MiR-30a inhibits subarachnoid fibrosis through inhibiting TRAF3IP2. (a and b) The mRNA and protein expression of miR-30a, TRAF3IP2, TGF-β1 and α-SMA from different groups (**P < 0.01 compared with the control group; ***P < 0.001 compared with the control group). (c) The content of collagen I and collagen III from different groups (***P < 0.001 compared with the control group. **P < 0.01 compared with the control group; *P < 0.05 compared with the control group).

Article Snippet: ELISA kit was used to detect the TGF-β1 level (EK0514, Wuhan Boster Biological Technology, Ltd, China), collagen type I level (CSB-E08084r, CUSABIO, China), and collagen type III level (CSB-E07924r, CUSABIO, China) following the protocols constructions.

Techniques: Expressing, Control

Journal: Arthritis Research & Therapy

Article Title: Heavy metal exposure reverses genetic resistance to Chlamydia -induced arthritis

doi: 10.1186/ar2610

Figure Lengend Snippet: The effect of prior exposure to mercuric chloride (HgCl 2 ) on serum anti- Chlamydia antibodies and anti-collagen antibodies in Chlamydia -induced arthritis. (a) HgCl 2 -exposed rats had significantly higher serum IgG1 anti- Chlamydia antibodies than non-exposed control rats ( P < 0.001). Control rats had slightly higher IgG2a anti- Chlamydia antibodies than the HgCl 2 -exposed rats ( P = 0.12). (b) HgCl 2 -exposed Brown Norway (BN) rats had higher levels of IgG antibodies to rat type II collagen than controls ( P < 0.01) (n = 8 in each group). O.D., optical density.

Article Snippet: The ELISA kit for the rat anti-type II collagen antibodies was from Chondrex (Redmond, WA, USA) and followed the protocol of the manufacturer.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production

doi: 10.3390/ijms222313089

Figure Lengend Snippet: Col I/III production from co-cultures with and without mechanically loaded myoblasts. ( A , D ) Relative mRNA expression of Col 1a1 and Col 3a1 in tenocytes. ( B , E ) Quantification of Col 1 content in tenocyte lysate by ELISA. ( C , F ) Quantification of Col I/III content and the ratio in the cell culture medium. ( G ) Immunofluorescence staining of Col I on tenocytes. Teno ctrl: tenocytes alone, Co ctrl: tenocytes co-cultured with myoblasts, Dyn: tenocyte co-cultured with dynamically loaded myoblasts, Stat: tenocyte co-cultured with statically loaded myoblasts. Data are represented as mean ± standard deviation. n = 3. Student’s t -test was performed when comparing Teno ctrl and co ctrl. One-way ANOVA with Tukey’s multiple comparisons was performed when comparing between Co ctrl, Dyn and Stat groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Collagen I and collagen III were assessed using Rat Collagen Type I ELISA kit (Cusabio, Wuhan, China, #CSB-E08084r) and Rat Collagen Type III ELISA kit (Cusabio, Wuhan, China, #CSB-E07924r) according to the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Standard Deviation